Abstract: The 129 Brassica campestris L. ssp. pekinensis（Lour.）Olsson DH lines grown in different seasons were used to identify glucosinolate profile and accumulation in leaves by HPLC analysis. Eight main glucosinolates were detected in all the accessions including 3 aliphatic glucosinolates，4 indolic glucosinolates and 1 aromatic glucosinolate. Principal component analysis showed Aliphatic glucosinolates，Gluconapin（NAP），Glucobrassicanapin（GBN）and Progoitrin（PRO）were the main glucosinolate profiles with the highest ratio of about 60.0% in Chinese cabbage，and 6 materials with a high content of glucosinolate were identified. Combined variance analysis showed that there were significant differences among varieties in aliphatic glucosinolates（NAP，GBN and PRO）. The accumulation of several indolic glucosinolates was affected greatly by environment，and a significant variance was appeared in different seasons. The aromatic glucosinolate，Gluconasturtiin（NAS）concentration varied significantly between two different seasons and season × variety interaction. Correlations analysis between individual glucosinolates showed that the accumulation of different glucosinolates was affected by each other，and correlation between NAP and total glucosinolate content，GBN and total glucosinolate，NAP and GBN was all significant respectively in the two seasons.

Key words: Brassica campestris L. ssp. pekinensis（Lour.）Olsson, glucosinolate, HPLC