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ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (5): 911-920.

• Vegetables • Previous Articles     Next Articles

Application of Common Bean Anthracnose Resistance Gene SCAR Markers in Snap Bean Disease Resistance Identification

GU Yu,HAN Qi-hou,WANG Wu-tai,LI Su-wen,SUN De-ling*,and WU Feng   

  1. (Tianjin Kernel Vegetable Research Institute,Tianjin 300382,China)
  • Received:2011-01-14 Revised:2011-04-11 Online:2011-05-25 Published:2011-05-25
  • Contact: SUN De-ling

Abstract: Five SCAR primer combinations(SCA1000,SH181100,SAB3400,SB12350 and SCF101072)of common bean anthracnose resistance gene were used to check the genome DNA of 143 snap bean germplasms. Meanwhile,their anthracnose resistance was identified by seedling inoculation method. The results indicated that the SCAR markers were found in 61 out of 143 germplasms,40 germplasms were detected having marker SCA1000,40 germplasms were detected having marker SCF101072,19 germplasms were detected having both marker SCA1000 and SCF101072. SCAR markers,SAB3400,SB12350 and SH181100,were not detected in all germplasms. Population resistance results revealed that 16 germplasms out of 143 tested germplasms were disease resistant. Among them,5 germplasms possessed two SCAR markers and disease resistance,3 germplasms possessed only one of the two SCAR markers with disease resistance. The results also showed that the content of Co-2 and Co-10 are very rich and distribute evenly in snap bean germplasms,except that the Co-2 content of is higher than Co-10 in purple bean pod. There are more resistance gene in green pods and You Dou. Numbers of owning both resistance gene and disease resistance are the most in You Dou.

Key words: snap bean, anthracnose, resistance gene, SCAR marker, seedling inoculation

CLC Number: