Abstract: Observation and histochemistry analysis were taken on all stages of epiphyllous ovule in Ginkgo biloba var. epiphylla development using resin semi-thin section technique. The results showed that：（1）The epiphyllous ovule apparent“gibbosity”at the stage of origin，its main construction were vascular bundle，parenchyma cells，and cavities；In the center of the“gibbosity”，there were a few starch grains，but a lot of starch grains were in the junction of“gibbosity”with leaf and distributed along the cell wall. （2）The development of epiphyllous ovule female gametophyte experienced free nuclei stage and cell wall formation stage；At free nuclei stage，the female gametophyte was liquid；At early cell wall formation stage，it was constituted by many big parenchyma cells，and there were no starch grains，lipid droplet and protein in them；Later，the starch grains became more；At the late wall formation stage of female gametophyte，the starch grains became more and bigger，and there was black lipid droplet surrounded cell wall. （3）At the stage of endosperm development，the cells accumulated starch grains and protein. The distribution of nutrients was centre-intensive，sparse-edge.（4）The differentiation of outer integument and meso-integument began at early free nuclei stage of female gametophyte；At the beginning of July，the inner integument appeared. （5）Between the meso-integument and inner integument，which was above the middle line，there was a“conoid”structure；Between the outer integument and meso-integument there were plenty starch grains.

Key words: Ginkgo biloba var. epiphylla, epiphyllous ovule, tissue structure, nutrients, developmental change