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ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (2): 353-353–360.

• Research Notes • Previous Articles     Next Articles

Distribution of 45S rDNA Sequence on Chromosomes of Five Species in Artemisia

LI Zhen,CHEN Su-mei,CHEN Fa-di*,WANG Hai-bin,TANG Fang-ping,LIU Zhao-lei,and FANG Wei-min   

  1. (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
  • Received:2010-06-28 Revised:2011-01-17 Online:2011-02-25 Published:2011-02-25
  • Contact: CHEN Fa-di

Abstract: Fluorescent in situ hybridization(FISH)was carried out to ascertain the distribution patterns of the 45S rDNA on chromosomes of five species in Artemisia. At mitotic metaphase in root tip cells,one pair of 45S rDNA loci was detected on chromosomes of A. absinthum Linn. Two pairs of signals were detected both in A. sieversiana Ehrhart ex Willd. and A. annua Linn. Four pairs of loci were detected both in A. abrotanum Linn. and A. japonica Thumb. A large part of sites were detected on the terminal regions on non-satellite chromosomes,besides one pair on subterminal regions,and the intensity of hybridization signals varied. Karyotype analysis based on FISH can not only provide stable and remarkable landmarks but also enrich the basic information on chromosomes.

Key words: Artemisia, 45S rDNA, FISH, karyotype

CLC Number: