Abstract: Cell suspension culture of Lilium regale Wils. were initiated from creamy-white nodular callus with loose structure. They were cultured in modified liquid MS medium（NH4NO3 with half-strength）supplemented with picloram 2.0 mg · L-1 and sucrose 30 g · L-1，and shook at 100 r · min-1 under dark condition at 25 ℃. About 10–20 days after，stably growing cell suspensioncultures were established. The growth pattern of the cultures was investigated and the optimal parameters were：0.5 g（FW）initial inoculum per 40 mL liquid medium，a sub-culture every 18 days，and 1/3 volume of old liquid medium left during subculture. Suspension cells regenerated plantlets on solid 1/2MS medium with 30 g · L-1 sucrose.

Key words: Lilium spp., Lilium regale Wils., cell suspension culture, plantlet regeneration