SCAR Markers Linked to Ms，a Genetic Male Sterile Gene in Chinese Cabbage

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (5): 757-762.

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SCAR Markers Linked to Ms，a Genetic Male Sterile Gene in Chinese Cabbage

LIU Zhi-yong，FENG Hui*，LI Cheng-yu，WEI Peng，and WANG Li-li

（Department of Horticulture，Shenyang Agricultural University，Shenyang 110866，China）

Received:2010-03-31 Revised:2010-05-04 Online:2010-05-25 Published:2010-05-25
Contact: FENG Hui

Abstract

Abstract: To identify molecular markers tightly linked to the male sterility gene Ms in Chinese cabbage, a BC1 population was developed from the cross between male sterile plants（MsMs）and fertile double haploid line ‘FH-1’（msms）. AFLP primers were screened for polymorphisms between the parents and two bulks representing fertile and sterile plants. Out of 512 primer combinations, 3 combinations gave polymorphic amplification patterns. These polymorphic amplicons were cloned and successfully converted to dominant SCAR markers, designated syau_scr02, syau_scr03 and syau_scr05. All the SCAR markers are located on one side of the Ms locus with a map distance ranging from 1.60 to 2.50 cM. These SCAR markers show high polymorphism between ‘AB01’ and 12 fertile inbred lines.

Key words: Brassica campestris L. ssp. pekinensis（Lour.）Olsson, genetic male sterility, AFLP, SCAR

CLC Number:

S 634.1

LIU Zhi-yong;FENG Hui;LI Cheng-yu;WEI Peng;and WANG Li-li. SCAR Markers Linked to Ms，a Genetic Male Sterile Gene in Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2010, 37(5): 757-762.

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SCAR Markers Linked to Ms，a Genetic Male Sterile Gene in Chinese Cabbage

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