Abstract: Leaf，petiole and pedicle explants of Pulsatilla koreana were cultured on MS mediumsupplemented with various concentrations of zeatin，kinetin or 6-BA combined with 0.05 mg · L^-1 IAA. After 6 weeks of culture，effects of cytokinin on adventitious shoot formation from explants were investigated. The highest frequency of shoot formation was obtained when petiole and pedicle explants
were cultured on MS + 0.5 mg · L^-1 zeatin + 0.05 mg · L^-1 IAA. Regenerated shoot were cut as several cluster or single plant with callus like tissue, and then transferred on MS + 0.5 mg · L^-1 zeatin + 0.05 mg · L^-1 IAA or MS + 1.0 mg · L^-1 6-BA + 0.05 mg · L^-1 IAA for shoot propagation and growth. When shoot reached more than 3 cm in length, they were transferred on to root induction medium. 93 percent of rooting ratio was observed in 1/2 MS + 1.5 mg · L^-1 NAA，after 42 days of culture. The rooted plantlets were acclimatized on mixture of North east mountain soil and perlite（2︰1）.

Key words: Pulsatilla koreana Nakai, adventitious shoot, cytokinin, rooting, transplanting