Abstract: Tospovirus，the only genus to infect plants in the Bunyaviridae family，can cause many crops and flowers to the serious disease in the tropics，the subtropics as well as the temperate zone area. This research surveyed the main flower fields of Yunnan Province in 2008. One sample with concentric rings spots and necrosis spots symptom was collected from Phalaenopsis amabilis in Kunming. By the biological techniques，electron microscope and ImmuStrip Test，it was confirmed that the plant was infected by Impatiens necrotic spot virus，belong to Tospovirus. Four pairs primers was used for RT-PCR，clone and sequencing，886 nts nucleic acid sequence of nucleocapsid protein，681 nts nucleic acid sequence of non-structure protein in RNA M and 1 048 nts fragment of RNA-dependent RNA polymerase（RdRp）were
obtained. By comparing with network database of NCBI BLAST and multiple alignment using biological information software DNAman，it was found that homology between the sample and Impatiens necrotic spot virus（INSV）reached higher than 90%. It was proved that the pathogens of the sample was Impatiens necrosis spot virus.

Key words: Phalaenopsis amabilis, impatiens ring spot disease, Impatiens necrosis spot virus, viral diseases