Abstract: Abstract: Based on the meiosis and microspore development of Lilium × formolongi ‘Raizan 1’, the length of flower bud and the colors of anther relative to the development stages were determined. The suitable media was selected for haploid callus induction of the ‘Raizan 1’, that was improved MS supplemented with 2,4-dichlunruphenoxyacetic（2,4-D）0.5 mg · L^-1 and kinetin（KT）4.0 mg · L^-1. The results showed the effects of sucrose concentration in the 30–90 g · L^-1 were not significant on the microspore callus induction. Microspores at early and mid-stage were more suitable for culture. Induction percentage of haploid calli were 44%, as bud length were 24–25 mm. Plantlet was obtained when haploid calli was transferred to the MS medium supplemented with 2.0 mg · L^-1 naphthaleneacctic acid（NAA）. The induction rate of plantlets were 100%，there into，plantlets of haploid were 43%.

Key words: Lilium × formolongi, microspore, haploid