Abstract: A pathogenic fungus causing leaf spot of Plectranthus parviflorus was isolated and characterized based on the morphological features, cultural characteristics and pathogenicity. By using polymerase chain reaction ( PCR) technique to amp lify rDNA2ITS of the isolate, we obtained a 523 bp DNA fragment, the result showed that rDNA-ITS sequences of the isolate and Myrothecium roridum shared 99% homology, which was compared in National Center for Biotechnology Information (NCBI) GenBank. Synthesized two identification results indicated that Myrothecium roridum was the pathogen causing black spot disease of Plectranthus parvif lorus. This is the first report of Myrothecium leaf spot caused by Myrothecium roridum on Plectranthus
parviflorus.

Key words: Plectranthus parviflorus, little spurflower black spot disease, Myrothecium roridum Tode exFr., ITS-rDNA sequencing