Study on in vitro Propagation of Endangered Plant Phyllitis japonia

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (9): 1373-1376.

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Study on in vitro Propagation of Endangered Plant Phyllitis japonia

GU De-feng^1*,LI Dong-sheng²,WANG Lei¹,QI Guang-xun¹,ZHAO He-xiang¹and DONG Ran¹

（¹College of Horticulture,Jilin Agricultural University,Changchun 130118,China; ²Dehui Agricultural development corporation，Dehui 130300，China）

Received:2008-05-07 Revised:2008-08-11 Online:2008-09-25 Published:2008-09-25
Contact: GU De-feng

Abstract

Abstract:

The sporophyllary leaves of Phyllitis japonica were used as explants to study the process of tissue culture rapid reproduction. The results showed that different inoculation types and culture methods significantly affected the spores germination and prothallus growth of Phyllitis japonica. The spores which sporangia were broken and cultured in liquid culture media germinated quickly. The prothallus propagated in MS culture medium without the plant growth hormone at a speed rate of 5 times. Pot culture of spraying 0.1% KH2PO4 upon prothallus is more suitable to translated from prothallus to spore.

Key words: Phyllitis japonica, Spore, Prothallus, Sporophyte, Rapid reproduction in vitro

CLC Number:

S 682.35

GU De-feng;LI Dong-sheng;WANG Lei;QI Guang-xun;ZHAO He-xiangand DONG Ran. Study on in vitro Propagation of Endangered Plant Phyllitis japonia[J]. ACTA HORTICULTURAE SINICA, 2008, 35(9): 1373-1376.

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Study on in vitro Propagation of Endangered Plant Phyllitis japonia

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