Abstract:

The composition and content of glucosinolates (GSs) in sprouts, leaves and edible roots of radish （Raphanus sativus L.） were analyzed by HPLC-MS. A total of eight GSs were identified in different tissues of radish, including five aliphatic GSs (4-methylsulfinyl-3-butenyl GS, 2-hydroxy-3-butenyl GS, ethyl GS, 4-methylthio-3-butenyl GS and 6-heptenyl GS) and three indole GSs (1-methoxyindol-3-ylmethyl GS, indol-3-ylmethyl GS, 4-hrdroxyindol-3-ylmethyl GS). Remarkable differences in glucosinolate profiles in sprouts, leaves and edible roots of radish were observed. The contents of total GSs in the sprouts and edible roots were higher than in the leaves. The major GS in edible roots and sprouts was 4-methylthio-3-butenyl GS, accounting for 75.5% and 71.5% of the total GSs, respectively. Indol-3-ylmethyl GS was the major GS in radish leaves, accounting for 57.1% of the total leaf GSs.

Key words: radish (Raphanus sativus L.), glucosinolate, composition and content, HPLC-MS