Identification of Differentially Expressed Genes by cDNA-AFLP Approach During Heat Stress in Tomato Leaves

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (7): 1011-1016.

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Identification of Differentially Expressed Genes by cDNA-AFLP Approach During Heat Stress in Tomato Leaves

LIU Zhi-yong^1,2, DU Yong-chen^1*, WANG Xiao-xuan¹, GUO Yan-mei¹, and GAO Jian-chang¹

（¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;²Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China）

Received:2008-03-05 Revised:2008-05-26 Online:2008-07-25 Published:2008-07-25
Contact: DU Yong-chen

Abstract

Abstract: In order to identify genes involved in heat shock responses in tomato, cDNA-AFLP approach was employed to identify genes differentially expressed in tomato leaves at high temperature. 768 primer combinations were used for selective amplification. 187 TDFs were selected for their differential expression under heat stress. Among the 187 TDFs, 153 TDFs were up-regulated and 34 TDFs were suppressed. 47 TDFs were cloned and sequenced. Compared with the publicly available databases, 34 TDFs presented some significant similarity with known plant sequences, whose functions are involved in gene regulation, transcription factor, signal transduction, stress defense，etc. 13 TDFs with low identity and no match to known genes may represent novel tomato genes.

Key words: tomato (Solanum lycopersicum), heat stress, cDNA-AFLP, differentially expressed genes

CLC Number:

S 641.2

LIU Zhi-yong;DU Yong-chen;WANG Xiao-xuan;GUO Yan-mei;and GAO Jian-chang. Identification of Differentially Expressed Genes by cDNA-AFLP Approach During Heat Stress in Tomato Leaves[J]. ACTA HORTICULTURAE SINICA, 2008, 35(7): 1011-1016.

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Identification of Differentially Expressed Genes by cDNA-AFLP Approach During Heat Stress in Tomato Leaves

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