Abstract:

The cryopreservation methods of Rehmannia glutinosa (Gaertn.) Libosch by vitrification and encapsulation-vitrification were studied. The results showed that: it was optimal cryopreservation condition for the two methods in the experimentation when the materials were trained at 4 ℃ low temperature for 5 d, then pre-cultured on the medium with DMSO and Acetamide for 3 d, loaded with 60 % PVS₂ for 25 min at room temperature and dehydrated with PVS2 for 30 min at 0 ℃. But compared with the materials cyropreserved by the two methods, the survival rate and regeneration rate by vitrification were both higher than those by encapsulation-vitrification. The survival rate (88.92 %) and the regeneration rate (64.29％) of the former were 1.38 times and 2.41 times higher than the latter, suggesting cryopreservation of Rehmannia glutinosa (Gaertn.) Libosch by vitrification is applicable to the germplasm conservation.

Key words: Rehmannia glutinosa (Gaertn.) Libosch, stem with bud, vitrification, encapsulation- vitrification, cryopreservation