Abstract: The L-galactono-1, 4-lactone dehydrogenase ( EC 1.3.2.3, GLDH) is a key enzyme that catalyzes the final step in the L-ascorbic acid synthetic pathway of plants. GLDH gene cDNA sequence was cloned with cDNA isolated from Brassica campestris ssp. chinensis‘Suzhouqing’. A 2 034 bp full-length cDNA sequence of GLDH was obtained using RT-PCR, nested PCR, 3 ’RACE and 5 ’RACE techniques.Further analysis on GLDH gene cDNA indicated that it encoded a peptide containing 601 amino acids. The amino acid sequence comparison with Brassica oleracea GLDH gene and Arabidopsis thaliana GLDH gene showed that identity was 98% and 90% , respectively. The sequence was accepted and released by GenBank (Accession number AY899298).

Key words: Brassica campestris ssp. chinensis Makino, L-ascorbic acid, L-galactono-1, 4-lactone dehydrogenase