Abstract: The CP genes were amplified by IC-PCR from CMV-HLJ and CMV-YN isolated from pumpkin in Heilongjiang and Yunan Provinces and cloned to pGEM-T vector. Analysis of nucleotide sequence showed that the length of CP genes of CMV-HLJ and CMV-YN was 657 bp, encoding 219 amino acid. The homology
of nucleotide and amino acid sequences of CP gene between these two isolates was 96.8% and 97.4% respectively. The homology of nucleotide and amino acid sequences of CP gene of these two isolates was higher than
90% and 97% respectively compared with six external isolates of CMV subgroupⅠ, and 66.4% and 73.4% respectively compared with another six external isolates of CMV subgroup Ⅱ, and higher than 89.5% and 93% respectively compared with ten Chinese isolates of CMV subgroup Ⅰ, and 68.3% and 73.5% respectively compared with two Chinese isolates of CMV subgroup Ⅱ. All these data showed that CMV-HLJ and CMV-YN belonged to CMV subgroupⅠ.

Key words: Pumpkin, CMV, IC-PCR, CP gene