Abstract: Chrysanthemum‘Qiyuehong’with small inflorescence was employed for study of cell suspension and plant regeneration. The results showed that MS(Murashige and Skoog) medium with supplement of 1.0 mg·L^-1 2,4-dichlorophenoxyacetic acid ( 2, 4-D) and 0.2 mg·L^-1 6-benzylamino purine ( 6-BA) could efficiently induce rapid-growing and loose calli. Obtained calli were subsequently cultured in liquid MS medium with 0.5 mg·L ^{- 1} 2, 4-D and 0.2 mg·L ^{- 1} 6-BA for establishing cell suspension system. The growth of suspension cultured cells showed a parabola curve, mainly characterized by lagged growth phase (0 - 2 d) , logarithmic growth phase (2 - 10 d) and stationary growth phase (10 d later) , respectively. In addition, pH value ofmedium decreased rapidly during the culture process. Highest plant efficiency was obtained on MS
medium with 0.2 mg·L ^-1 kinetin (KT) and 0.2 mg·L ^{- 1} 2, 4-D. Low temperature treatment at 4℃ for 2 hours could enhance the growth of cell, and increase the planting efficiency as well. However, the p lant efficiency decreased with increasing of subculture times. Calli with a diameter of 0.2 cm were obtained in one month. When theywere transferred to MS medium containing 2.0 mg·L ^-1 6-BA and 0.1 mg·L ^-1 naphthaleneacetic (NAA) and subcultured for 4 times, shoots differentiated from the calli. Rooting plantlets were induced on MS with 0.5 mg·L ^-1 NAA.

Key words: Dendranthema ×grandiflorum, Cell suspension culture, Plant regeneration