Construction and Identification of a cDNA Expression Library from Dendrobium nobile

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 895-897.

• 研究报告 • Previous Articles Next Articles

Construction and Identification of a cDNA Expression Library from Dendrobium nobile

Zhuang Junping;Huang Shengqin;Pan Shuqun;Ye Qingsheng^*

(College of Life Science, South China Normal University, Guangdong Key Laboratory of Biotechnology for Plant Development,Guangzhou, Guangdong 510631, China)

Received:2005-09-14 Revised:2005-12-01 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: A cDNA expression library from the flower bud of Dendrobium nobile was constructed into pcDNA3.0 eukaryotical expression plasmid used SMART^TM cDNA library construction protocol. The result showed that the cDNA expression library contained 3.02 ×10⁵ pfu, the size of the inserts ranges from 0.5 kb
to 2.5 kb, and the percentage of positive clones was almost 100%. All of the above mentioned have answered for the general requirements of a cDNA library. This provides a base for cloning of important genes related to
flower bud differentiation in Dendrobium nobile.

Key words: Dendrobium nobile, Flower bud, cDNA expression library

Zhuang Junping;Huang Shengqin;Pan Shuqun;Ye Qingsheng. Construction and Identification of a cDNA Expression Library from Dendrobium nobile[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 895-897.

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Construction and Identification of a cDNA Expression Library from Dendrobium nobile

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