In Vitro Culture of Unfertilized Ovules in Phalaenopsis

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 891-894.

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In Vitro Culture of Unfertilized Ovules in Phalaenopsis

Wu Chenghou^{1, 2, 3*};Pan Yishan¹;Luo Kaimei¹;Ye Xiulin^2*;Liang Chengye²

(¹Department of Biology, Zhangzhou Normal College, Zhangzhou, Fujian 363000, China; ²South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; ³Guangzhou Institute of Landscape and Garden, Guangzhou, Guangdong 510405, China)

Received:2005-11-15 Revised:2006-04-27 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: The effects of medium composition on the in vitro culture of unfertilized ovules of a Phalaenopsis hybrid were studied. The results showed that the megasporocytes of the hybrid were capable of undergoing meiosis to form bi-nucleate embryo sacs in vitro by ovule cultures on the medium of VW + BA 0.2 mg·L ^{- 1} + NAA 0.2 mg·L ^{- 1} + sucrose 2.0% + phytagel 0.3%. On the medium of VW + BA 0.2 mg·L ^{- 1} +NAA 0.2 mg·L ^{- 1} + sucrose 2.0% + phytagel 0.3% + coconut milk 10% , the megasporocytes would develop into 4-nucleate embryo sacs or embryogenic cell masses. These embryogenic cell masses may develop into haploid plants.

Key words: Phalaenopsis, Ovule, Embryogenic cell masses, Tissue culture

Wu Chenghou;;;Pan Yishan;Luo Kaimei;Ye Xiulin;Liang Chengye. In Vitro Culture of Unfertilized Ovules in Phalaenopsis[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 891-894.

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In Vitro Culture of Unfertilized Ovules in Phalaenopsis

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