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ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 887-890.

• 研究报告 • Previous Articles     Next Articles

Effects of Plant Growth Regulators on the Rhizomeps Multiplication and Differentiation in Cymbidium goeringii with Verge Line Pattern Leaves

Shi Lejuan;Zhang Fang;Zhang Shiliang; Bu Yanfeng;Ye Xiaowei   

  1. (Department of Horticulture, Zhejing Universty, Hangzhou, Zhejiang 310029, China)
  • Received:2005-10-08 Revised:2005-12-12 Online:2006-08-25 Published:2006-08-25

Abstract: The rapid multiplication system of Cymbidium goeringii‘Lüyun’with verge line pattern leaves by protocorm like-body was established. Effects of plant growth regulators on multiplication and differentiation of rhizomes were studied. 1 /2MS solid medium was suitable for subculture and preservation of the rhizome. The optimal medium for multiplication and growth of rhizomes was MS + 6-BA 1.0 mg·L - 1. IBA was more effective to the growth of rhizomes than NAA with increasing of auxin concentration (0 - 3 mg·L - 1 ).The optimum medium for differentiation of rhizomes was 1/2 MS supplemented with 6-BA 1.0 mg·L - 1 and IBA 1.0 mg·L - 1 , which regeneration rate of shoots was as high as 36.9%. Dasheen paste was better to the multiplication of rhizomes than coconut milk and banana mud added to the culture medium. Single conglomeration with 3 - 5 rhizomes was optimum by the way of broking off with fingers and thumb. Verge line pattern on the leaf could be stably inherited.

Key words: Cymbidium goeringii, Verge line pattern, Protocorm like-body, Rhizome, 6-BA, IBA