Studies on Optimizing Production of Wasabi Plantlet

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 876-878.

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Studies on Optimizing Production of Wasabi Plantlet

Cui Cui;He Fengfa^*;Zhou Qingyuan;Wang Jichun;Huang Yuanxin

(College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China)

Received:2005-08-22 Revised:2005-11-14 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: The study showed that the concerntration of 6-BA, NAA and Vitamin C affected plantlet propogation coefficients ofWasabi through optimization regression analysis. As a result, the suitable combination was selected, the proliferation coefficient might increase to 6.8380 when treated byMS medium with 6-BA
1.2504 mg/L and Vitamin C 1.9668 mg/L. In rooting culture, 1 /2MS medium or 1 /2MS with 0.01mg/L NAA were suitable for root development and growth, and rooting frequency reached above 90%. In acclimatization, the results showed that strong plantletswithout rootingwere fit for culture out of door with around 80% rooting frequency, therefore, production cost of wasabi seedlings decreased greatly without rooting phases.

Key words: Wasabi, Tissue culture, Regression design

Cui Cui;He Fengfa;Zhou Qingyuan;Wang Jichun;Huang Yuanxin. Studies on Optimizing Production of Wasabi Plantlet[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 876-878.

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Studies on Optimizing Production of Wasabi Plantlet

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