Abstract: Sucrose less than 4% in medium could not stimulate formation of rhizome for Stachys floridana Schuttl. ex Benth. on the medium without revulsant. There was no significant revulsive difference between MS medium and MB medium with 10% sucrose. Glucose could induce significantly more rhizome than sucrose at the same concentration level, but could not make the increasing of gross fresh weight of rhizome. Every plant of Stachys floridana Schuttl. ex Benth. would be induced to produce 3.33 pieces and 0.45 g of rhizomes on
MS medium containing 4% sucrose, 8.0 mg·L ^{- 1} 6-BA and 10 mg·L ^{- 1} SA, which was significantly better than other combinations. The change of ploidy level was not observed in the induced rhizomes according to the result of determination by flow cytometry.

Key words: Stachys floridana Schuttl. ex Benth., Rhizome, Revalsant, Inducement in vitro, Flow cytometry