Application of AFLP Markers in Assisted Selection of Cabbage ( Brassica oleracea ) Dominant Male Sterility High Generation Backcrosses

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (3): 539-543.

• 研究论文 • Previous Articles Next Articles

Application of AFLP Markers in Assisted Selection of Cabbage ( Brassica oleracea ) Dominant Male Sterility High Generation Backcrosses

Huang Heyan; Zhang Yanguo;Deng Bo;Lou Ping;Wang Xiaowu^*

( Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2005-07-11 Revised:2005-11-21 Online:2006-06-25 Published:2006-06-25

Abstract

Abstract: Three different genotypes (MsMs, Msms and msms) generated by selfing an individual after 8 generation by backcrossing the line 02-12 into a dominant male sterile line DGMS79-399-3 were rapidly screened using infrared fluorescence-labeled AFLP technology and Li-cor gene sequencer. In total, 17 AFLP markers were identified by screening 512 primer combinations. 11 markers covering 21 cM linked to the dominantmale sterility gene were found in 95 individuals of BC₈ population, while the other 6 markers covering 9 cM were genetic carry-over without linkage to the gene. Three promisingmale sterile individualswere selected using the marker information, which had both less genetic drag and carry-over for further backcross.

Key words: Brassica oleracea, Dominantmale sterility, AFLP, Marker-assisted selection

Huang Heyan;Zhang Yanguo;Deng Bo;Lou Ping;Wang Xiaowu. Application of AFLP Markers in Assisted Selection of Cabbage ( Brassica oleracea ) Dominant Male Sterility High Generation Backcrosses[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 539-543.

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Application of AFLP Markers in Assisted Selection of Cabbage ( Brassica oleracea ) Dominant Male Sterility High Generation Backcrosses

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