Abstract: The regeneration system of Lavandula angustifolia ‘Munstead’ in vitro was studied with leaves as explants, and the results showed the optimal medium for callus induction was MS + 2,4-D 0.1 mg/L + 6-BA 0.5 mg/L with the induction frequency of 100%. Liquid suspension solid culture can achieve adventitious bud differentiation rate of 92.5% and 6.6 bud per callus. Best bud multip lying medium which was MS medium supplemented with NAA 2.0 mg/L and 6-BA 0.5 mg/L and IAA 1.0 mg/L was sifted through orthogonal experiment with multip lying coefficient of 8.7. The rooting of rootless bud could be accomplished directly in the bud multip lying medium with rooting frequency of 100%.

Key words: Lavandula angustifolia, Liquid suspension-solid culture, Regenerated plantlet