Interspecific Hybridization between Brassica oleracea L. var. botrytis andBrassica spinescens via Asymmetric Somatic Protoplast Fusion

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (6): 1039-1044.

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Interspecific Hybridization between Brassica oleracea L. var. botrytis andBrassica spinescens via Asymmetric Somatic Protoplast Fusion

Yao Xingwei;Liu Fan;Yun Xingfu;Zhao Hong;Ryschka U;Schumann G

(¹Agricultural College, Inner Mongolia Agricultural University, Huhhot 010019, China; ²Beijing Vegetable Research Center, Beijing 100089, China; ³ Institute of Horticultural Crops of Federal Center forB reeding Research on Cultivated Plants, Neuer Weg 22/23, Quedlinburg D206484, Germany)

Received:2005-03-04 Revised:2005-04-13 Online:2005-12-25 Published:2005-12-25

Abstract

Abstract: Asymmetric protoplast fusion between Brassica oleracea L. var. botrytis and B. spinescens was taken for transferring of wild resistant traits into B. oleracea. B. spinescens possesses desirable characteristics such as high photosynthetic efficiency, resistance to the white rust ( caused by Albugo candida) , aphid
and alternaria blights ( caused by Alternaria brassicae) and tolerance to salt. PEG ( polyethyleneglycol) induced asymmetric fusions were performed between mesophyll p rotop lasts of B. spinescens irradiated with UV and hypocotyl protoplasts of B. oleracea L. var. botrytis, and 379 regenerated plants were obtained. Peroxidase isozyme ( POX) analysis was taken in 120 plants, and 23 of them showed hybrid characters that were confirmed by the RAPD marker detection. The relative nuclearDNA content of the hybridsmeasured by flow
cytometry ( FCM) showed that all of the 23 analyzed plants had a DNA content from tetraploidy to octoploidy. Influence of UV doses had been tested, and results showed that the maximum UV dose for B. spinescens was 0.0750 J·cm^{- 2}.

Key words: Brassica, Interspecific hybrid, Asymmetric somatic fusion, Protoplast culture, Hybrid identification

CLC Number:

S 635.3

Yao Xingwei;Liu Fan;Yun Xingfu;Zhao Hong;Ryschka U;Schumann G. Interspecific Hybridization between Brassica oleracea L. var. botrytis andBrassica spinescens via Asymmetric Somatic Protoplast Fusion[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1039-1044.

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Interspecific Hybridization between Brassica oleracea L. var. botrytis andBrassica spinescens via Asymmetric Somatic Protoplast Fusion

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