Abstract: Five cold stress gene fragments were isolated through cDNA-AFLP from Volvariella volvacea and were designated as VC1, VC2, VC3, VC4 and VC5 respectively. DNA sequence results indicated that these five DNA fragments had 247 bp, 219 bp, 172 bp, 350 bp and 171 bp respectively. BLAST search results showed that VC1, VC2, VC3, VC4 and VC5 had no significant homologous nucleotide sequences found in databases. Postulated p rotein sequences of VC1, VC2 were homologous with the hypothetical protein of
Neurospora crassa and the putative AMP deaminase of Oryza sativa, espectively. Postulated protein sequences of VC3, VC4 were homologouswith the glycogen synthase kinase three alpha in Danio rerio and the hypothetical protein in Leuconotocm esenteroides respectively. But postulated protein sequence of VC5 had not found the significant homologous p rotein sequence in the databases yet.

Key words: Volvariella volvacea, cDNA-AFLP, Cold stress gene