Studies on the Changes of Endogenous Hormones during the Course of Regeneration from Allium fistulosum L. Mature Embryos Culture

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (02): 318-320.

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Studies on the Changes of Endogenous Hormones during the Course of Regeneration from Allium fistulosum L. Mature Embryos Culture

Sheng Yanping¹;Yang Jianping¹;Zhang Song¹;Qi Xianlei²;Li Xincheng³

(¹College of Horticulture, Shandong Agricultural University, Tai'an 271018, China; ²College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China; ³ Tai'an Institute of Vegetable Basket, Tai'an 271018, China)

Received:2004-04-01 Revised:2004-06-21 Online:2005-04-25 Published:2005-04-25

Abstract

Abstract: The callus induction and p lant regeneration from Allium fistulosum L. mature embryos culture were studied in A. f istulosum L. and the endogenous hormone changes in this course were analyzed. The callus growth rate reached the highest point at the 6 th day after culturing, which might be caused by the maximum concentration of ZR at the 3 rd day. ABA was a negative regulation factor in the callus induction. The concentration of GA₃ during callus induction wasmuch higher than that in the multip lication course, which indicated GA₃ was very important in the differentiation period. The appropriate proportion between 6-BA and NAA was beneficial to the shoot differentiation of A. fistulosum L. , but there was side effect to shoot regeneration if CTK concentration was too high.

Key words: Allium fistulosum L., Mature embryos, Callus induction, Differentiation, Endogenous hormones

CLC Number:

S633.1

Sheng Yanping;Yang Jianping;Zhang Song;Qi Xianlei;Li Xincheng. Studies on the Changes of Endogenous Hormones during the Course of Regeneration from Allium fistulosum L. Mature Embryos Culture[J]. ACTA HORTICULTURAE SINICA, 2005, 32(02): 318-320.

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Studies on the Changes of Endogenous Hormones during the Course of Regeneration from Allium fistulosum L. Mature Embryos Culture

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