Abstract: pBI1212based vector pZM 612 containing an antisense full2length 2b gene of CMV2BG was constructed and introduced into Nicotiana tabaccum var. SR1 , via Agrobacterium tumef aciens2mediated leaf2disc transformation. The regenerated plants were screened by PCR , and further verified by PCR-Southern and one-step RT-PCR. The results indicated that antisense 2b gene of CMV2BG had been integrated into the tobacco chromosomes ,and expressed at transcriptional level . The resistance of transgenic plants was observed after viral infection , indicating the mediation of resistant to CMV2BGby the antisense 2b gene. The antisense 2b gene of CMV2BG (pZM 612) was also introduced into Lycopersicon escuentum var. Hongmanao 213 , and PCR positive transgenic tomato plants were obtained.

Key words: CMV, Antisense 2b gene, Transgenic tomato