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ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (1): 25-28.

• 研究论文 • Previous Articles     Next Articles

Cloning and Sequencing of cDNA Fragments Differentially Expressed betweenAborted and Normal Development Embryo in Litchi

Zhang Yishun1, Xiang Xu2 Fu Jiarui1, Huang Shangzhi1   

  1. (1Life Science School, Zhongshan University, Guangzhou 510275, China; 2Pomology Institute, Guangdong Academy of Agricultural Science, Guangzhou 510640, China)
  • Received:2003-05-22 Revised:2003-08-25 Online:2004-02-25 Published:2004-02-25

Abstract: The suppressive subtraction hybridization (SSH) technique was used to clone the cDNA fragments differentially expressed between aborted and normal development embryo of ‘Guiwei’varieties. One positive differential expressing cDNA library were constructed using cDNAs of normal development embryo of Guiwei as driver and the cDNAs of aborted embryo of Guiwei varieties as tester. This cDNA library indicated the differential expressing
cDNA fragments in Guiwei aborted embryo and Guiwei normal development embryo. By Virtual Northern blots, 3 positive clones were obtained. Subsequently sequencing analysis showed the 3 clones were corresponding 3 knew gene sequence and the 3 cDNA fragments were first reported in litchi varieties.

Key words: Litchi, Aborted embryo, Suppressive subtraction hybridization (SSH), Virtual Northern blot

CLC Number: