Abstract: Taking MS as basic media , adopting 6-BA , NAA , KT of different concentration compoundings to induce callus from cotyledons and hypocotyls of Cleome spinosa Jacq. , the result showed that the mediumof MS +6-BA 0.5 mg/ L + NAA 0.5 mg/ L + KT 0.5 mg/ L was the best to induce callus from cotyledons with a rate of 100 %frequency , and the highest rate of hypocotyl callus inducement was 93 %. And the differentiation medium MS + 6-BA 2 mg/ L + NAA 0.05 mg/ L was the best to induce buds and had a 100 % plant regeneration. And it was for rooting media , MS + NAA 0.1 mg/ L ,to produce roots from seedings. Taking the stems with single bud as explants on MS + 6-BA 1 mg/ L + NAA 0.05 mg/ L , it was found that there are lots of buds after a month.

Key words: Cleome spinosa Jacq., Culture in vitro, Plant regeneration