Abstract: Taking a constant cell suspension systemof carrot ( Daucus carota L. ) variety -Xin Touxinhong as material , the effects of basic medium , sucrose and PO₄^3- concentrations on lycopene synthesis activity of carrot suspension cells were studied by measurement and significant analysis on lycopene content of fresh cells per unit weight (in terms of light absorption values at 470 nm) among different treatments. The results showed that the lycopene content in carrot suspension cells under MG₅ medium was significantly higher than those under the other media , MG₅ was the most favorable basic medium for lycopene synthesis of carrot cells among MG₅ , ER , B₅ and White ; on the basis of MG₅ medium , the lycopene contents under 100.0 - 120.0 g·L^-1 of sucrose concentration among 7 treatments and 30.0 - 50.0 mmol·L^-1 of PO₄^3-concentration among 13 treatments were very significantly higher than those of the other corresponding treatments , and better for lycopene synthesis of carrot cells

Key words: Daucus carota L., Sucrose concentration, PO₄^3- concentration, Lycopene synthesis, Cell suspension system