https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (5): 540-544.

• 研究论文 • Previous Articles     Next Articles

Amplified Resistance Phytophthora Blight Gene Analogs from Capsicum annuumGenomic DNA

Yi Tuyong1,2;Xie Bingyan1;Zhang Baoxi1;Gao Bida2   

  1. (1 Institute of Vegetables and Flowers , Chinese Academy of Agricultural Sciences , Beijing 100081 , China ;2 College of Plant Protection , Hunan Agricultural University , Changsha 410128 , China)
  • Received:2003-02-12 Revised:2003-07-25 Online:2003-10-25 Published:2003-10-25
  • Contact: Xie Bingyan

Abstract: Numerous fragments were cloned from 9 different resistant/ susceptible pepper ( Capsicum annuum)varieties to Phytophthora blight , using a PCR approach with degenerate primers designed from conserved motifs found in a number of plant resistance genes. After sequencing and homology analysis , 8 RGAs amplified by primer B were high identified with N , RPS2 , L6 and belonged to broad-spectrum RGA , 6 RGAs amplified by primer C were high identified with Cf2 , Cf9. 14 RGAs amplified by both primer B and primer C were relative to function of resistancee to Phytophthora blight . RGAs derived from susceptible lines were also associated with function of resistant to Phytophthora blight , which was accorded with reported viewpoint that resistant minor QTLs derived from susceptible parents. These pepper RGAs could be regarded as candidate sequences of disease resistance genes in Capsicum annuum using cloning resistant genes and molecular markers.

Key words: Capsicum annuum, Phytophthora blight, Resistance gene analogs

CLC Number: