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ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (4): 485-486.

• 研究报告 • Previous Articles     Next Articles

Discrimination of the Genomes in BC1 Progeny of Asiatic Lfly and Oriental LilyUsing GISH

Zhou Shujun
  

  1. (College ofForesty,Shandong Agricultural University,Ta’an 271018,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-08-25 Published:2003-08-25

Abstract: Genomic in situ hybIridization(GISH)was used to discriminate the diferent genomes in the BC1 progeny of Oriental lily and Asiatic lily. We used gen c DNA of Oriental Lily as probe an d HS sperm DNA or genomic DNA of Asiatic Lily as block DNA for chromosome painting in the present paper. 卟e result clearly showed the diference between the two genomes of Oftental Lily an d Asiatic Lily in their BC1.This confirmed that GISH could be used not only to identify the successfulness of the cross between Oriental Lily and Asiatic Lily but also to analyze the chromosome crossover or recombination between the two diferent genomes.

Key words: Asiatic Lily, Oriental Lily, Genomic in situ hybridization(GISH), Iryotype

CLC Number: