Propagation of Bublets in Lium ‘Casa Blanca’ Using Bioreactor

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (4): 479-781.

• 研究报告 • Previous Articles Next Articles

Propagation of Bublets in Lium ‘Casa Blanca’ Using Bioreactor

Lian Meilan，Piao Xuanchun ，and Paek Kee Yoeup

Agricultara College of Yanb， 133400，China； Research lhelk,telop．merit of A &．anced Horticultural Technology， National Universy，Cheon 361—763，Korea)

Received:1900-01-01 Revised:1900-01-01 Online:2003-08-25 Published:2003-08-25

Abstract

Abstract: T0 investigate possibility of bulblet production of f2ium ‘Casa Blanca’ in bioreactors， bioreac．tor and solid culture methods were studied． Increased bulblet growth was observed in bioreactor culture than solid cultures．Bulblet growth was 16．5 fold in solid culture and it Was 22．4 fold in bioreactor culture． Culture density in bioreactor culture Was studied and 4o0 bulblets were suitable for 5 L bioreactor and from these cultures we obtained 295 bulblets of size≥ 1．1 g． Stem emergence of bulblets obtained from both cultures showed that 90％ 0f bulblets(≥1．1 g)from bioreactor culture and 65％ from solid cultures(i>2．1 g)developed stem respectively．

Key words: Lilium ‘Casa Blanca’, Bioreactor, Tissue culture, Virus．free

CLC Number:

S 68

Lian Meilan;Piao Xuanchun;and Paek Kee Yoeup. Propagation of Bublets in Lium ‘Casa Blanca’ Using Bioreactor[J]. ACTA HORTICULTURAE SINICA, 2003, 30(4): 479-781.

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Propagation of Bublets in Lium ‘Casa Blanca’ Using Bioreactor

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