ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (6): 542-546.
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Wu Daofan and Song Ming
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Abstract: Cabbage seeds of different vigor degree were obtained by accelerated aging. Some methods ,Including treatments of imbibition/ dryback , mannitol gibberellin ( GA) , polythyleneglyool ( PEG) , CaCl2 , KH2PO4 , ascorbic acid (AsA) , were were investigated to enhance the forgoing seed vigor by L9 (34) orthogonal experiment. Then the changes of cabbage seed at physiological and biochemical level , cell level and structure of tissue cells were investigated. The results indicated that : ①All the 7 methods could improve seed vigor ofcabbage. Seed vigor was significantly enhanced after seed were treated by all the best effect combination , and PEG, GA treatment was the best one , the difference between PEG, GA and others methods was significant . ②Some physiological and biochemical changes were obvious : protease , dehydrogenase , SOD , CAT and POD were reduced significantly ; relative elelctive conductivity ( REC) and the content of MDA in seed were increasedsignificantly. Analysis indicated that they were correlated significantly with vigor , they were the main reason of change of seed vigor , and were reliable indexes of measuring seed vigor (at physiological and biochemical level) .③Karyotype of root tip chromosome showed that the chromosome did not change clearly before and after improving seed vigor. ④Observation on the ultrastructure of the radicle cell of cabbage seed indicated that ultrastructurechanged with seed vigor reduced , the lower seed vigor , the clearer the change. When seed vigor was improved by all kinds of methods , ultrastructure of cell would be recovered at different degree.
Key words: Cabbage seed, Seed vigor, Methods, Mechanisms
Wu Daofan and Song Ming. Studies on Methods and Mechanisms of Improving Vigor in Cabbage Seed(Brassica oleracea L. var. capitata)[J]. ACTA HORTICULTURAE SINICA, 2002, 29(6): 542-546.
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