Abstract: Taking MS、1/ 2MS as basic media, adopting 6-BA、NAA、and 2, 4-D of different concentration composition to conduct t issue culture and plant regeneration from hypocotyl of lettuce, the result showed that medium of MS+ 6-BA 0. 5 mg/L+ 2, 4-D 1mg/L+ NAA 0. 1mg/ L , was the best to induce callus from hypocotyl, the rate of callus is 94%; and the differentiat ion medium, MS+ 6-BA 1. 0mg/ L + NAA 0. 05mg/ L, was the best to induce buds from callus and had a 100% plant regenerat ion rate; and it was for rooting media, 1/ 2MS+ NAA 0. 5 mg/ L, to induce root ing from seedlings.

Key words: Lettuce (Lactuca sativa), Hypocotyl, Callus tissue, Plant regeneration