Phylogenetic Relationship among Nine Prunus Species Based on Random Amplified Polymorphic DNA

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (3): 218-223.

• 研究论文 • Previous Articles Next Articles

Phylogenetic Relationship among Nine Prunus Species Based on Random Amplified Polymorphic DNA

Ruan Ying¹, Zhou Puhua¹, and Liu Chunlin²

(¹ Science School of Hunan Agriculture University, Changsha 410128, China; ² Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agriculture University, Changsha 410128, China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-06-25 Published:2002-06-25

Abstract

Abstract: In this study, nine species of Prunus (20 materials) were analyzed using random amplified polymorphic DNA markers. 24 primers were screened from 45 arbitrary 102mer primers , and a total of 326 DNA bands were amplified , 311 of which (95. 4 %) were polymorphism. According to the data , a tree diagramof phylogenetic relationship was constructed using UPGMA in NYSYS. The results showed that : 1) P. ussuriensis was a variety of P. salician , not a independent species ; 2) P. simonii was a hybrid of P. salician and P. armaniaca and was closely related to P. armaniaca ; 3) P. domestica was a offspring formed by naturally crossing between P.cerasifera and P. spinosa.

Key words: Prunus, RAPD analysis, Phylogenetic relationship

CLC Number:

S 662. 3

Ruan Ying;Zhou Puhua;and Liu Chunlin. Phylogenetic Relationship among Nine Prunus Species Based on Random Amplified Polymorphic DNA[J]. ACTA HORTICULTURAE SINICA, 2002, 29(3): 218-223.

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Phylogenetic Relationship among Nine Prunus Species Based on Random Amplified Polymorphic DNA

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