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ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (1): 69-71.

• 研究报告 • Previous Articles     Next Articles

Molecular Identification of Main Cultivars of Torreya grandis by RAPDMarkers

Tan Xiaofeng, Hu Fangming, Zhang Dangquan, Zhou Xuhui, and Yang Wei
  

  1. (The Key laboratory of Non wood Forest Tree Breeding and Cultivation of Forestry Ministry, Zhuz hou 412006)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

Abstract: The endosperm DNA samples of the seeds of a same individual plant of eight main cultivars (clones) of Torreya grandis are analyzed with RAPD technology by using 23 primers that have distinct polymor phism。The result indicated that there was a relatively large difference between the eight varieties at the level of DNA molecule; the marker genotype of varieties was established according to particularly isolated bands in the experiments; the clone varieties of Torreya grandis can be identified at the level of DNA molecule according to the marker genotype; the eight varieties of Torreya grandis were analyzed systematically with a assembling analysis tech nology, and the analyt ic result tallied with the two classes of tradit ional morphological classif icat ion on the whole.

Key words: Torreya grandis, Cultivars, RAPD marker, Molecular identificat ion, Molecular classification

CLC Number: