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ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (4): 345-347.

• 研究报告 • Previous Articles     Next Articles

Research on Outer Bottle Rooting Technique of Fruit Seedling in Vitro with Filter-paper Bridge

Sun Zhongxu, Liu jing, Wang Yujun, Zhang Yanyi, and Qiu Zhilin   

  1. (1 Department of Horticulture , Shandong Agricultural University , Tai’an 271018; 2 Tai’an City Forest Institute , Tai’an 271018)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-08-25 Published:2001-08-25

Abstract: The rooting technique of fruit tissue culture system with filter paper bridge were studied in this experiment. The results indicated that the rooting rates of Zhumei crabapple and strawberry with filter-paper bridge were 20 %
and 4. 1 % more than those of their controls respectively ; the rooting rate of cherry stock was same as that of its control , but the rooting time was decreased to 3 - 10 days ; the transplanting survival rates of these three kinds of fruit tree plantlets all increased up to 93 % , the survival rates of Zhumei crabapple , strawberry and cherry stock were increased by 16. 1 % , 21. 4 % and 14. 9 % respectively , compared with their controls ; the root vigor areas of Zhumei crabapple , Strawberry and cherry stock were increased by 6. 9 % , 11. 54 % and 7. 5 % , compared with their controls ; the thickness of epicuticle , hypodermis and pajisade tissue and the leaf stoma density of Zhumei crabapple were increased by 45. 8 % , 50. 3 % , 47. 6 % and 2. 5 % respectively , compared with its control. This method is simple and convenient. The cost of tissue culture could be cut down 75 % than traditional rooting methods.

Key words: Tissue culture seedling, Filter-paper bridge, Rooting, Rooting rate, Survival rate

CLC Number: