Cloning and Sequencing of Antifreeze Protein Gene in Daucus carota var. sativus Hoffm Deutschl

ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (2): 173-174.

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Cloning and Sequencing of Antifreeze Protein Gene in Daucus carota var. sativus Hoffm Deutschl

YinMing'an¹, Cui Hongwen¹, Fan Daiming², and Guo Li¹

(¹Northwest Sci-Tech University of Agricultural and Forestry , Yangling 712100; ²The Fourth University of Military Medicine, Xi'an 710032)

Received:1900-01-01 Revised:1900-01-01 Online:2001-04-25 Published:2001-04-25

Abstract

Abstract: Three carrot cultivar Wuzhong Ningxia, Huaxian Shaanxi and Hanzhong Shaanxi were used as test material and antifreeze protein gene (afp) of Chinese carrot (Daucus carota var. sativus Hoffm Deutschl) was cloned
and sequenced by PCR (polymerase chain reaction) . Obtained sequence was compared with that of British carrot (Daucus carota var. autumn King) . There were 36 different bases in 1004 nucleotides (3. 6%) between the two varieties. Among the different bases there were 21 nonsense mutations and 15 sense mutations. According to sense mutations, homology was 98. 5%.

Key words: Carrot (Daucus carota var. sativus Hoffm Deutschl), Antifreeze protein, Molecular biology

CLC Number:

YinMing'an;Cui Hongwen;Fan Daiming;and Guo Li. Cloning and Sequencing of Antifreeze Protein Gene in Daucus carota var. sativus Hoffm Deutschl[J]. ACTA HORTICULTURAE SINICA, 2001, 28(2): 173-174.

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Cloning and Sequencing of Antifreeze Protein Gene in Daucus carota var. sativus Hoffm Deutschl

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