Abstract:

This study based on ultra performance liquid chromatograph（UPLC）for rapid determination method of polyphenols and its content in the sponge gourd to supports luffa enzymatic browning of follow-up studies to provide technical. The liquid-chromatographic separation was performed on Agilent ZORBAX Eclipse Plus C18（2.1 mm × 100 mm，1.7 μm-Micron）column kept at 32 ℃，using methanol/acetic acid/water mixture as the mobile phase with a flow rate of 0.25 mL · min^-1 through gradient elution：0–0.5 min，95% B；0.5–17.5 min，95%–67.5% B；17.5–18 min，67.5%–95% B. The detection wavelength was set at 283 nm. Results showed that 14 polyphenols standard was completely separated within 18 min where detecting thirteen poly samples. The determination coefficients were higher than 0.999 and linear range was from 0.05 to 4 mg · L^-1. The limits of quantification range from 0.004 to 0.049 mg · mL^-1. Recoveries achieved from the luffa ranged from 95.70% to 106.89%（RSDs，0.32%–4.71%）. The precision，reproducibility and stability were satisfactory with the relative standard deviations（RSDs）less than 5%. Gentisic acid as the main polyphenols，followed by chlorogenic acid，dopamine，L-tyrosine，finally the pyrobitumengallic acid，protocatechuic acid，vanillic acid，catechin，epicatechin，p-hydroxy benzoic acid and clove acid as well as the 4-methyl catechol，coumaric acid.

Key words: luffa, polyphenols, ultra performance liquid chromatography