Cloning and Expression Analysis of Tomato LeLACmiR397 Gene

doi:10.16420/j.issn.0513-353x.2014-1079

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (7): 1285-1298.doi: 10.16420/j.issn.0513-353x.2014-1079

• Vegetables • Previous Articles Next Articles

Cloning and Expression Analysis of Tomato LeLACmiR397 Gene

ZHAO Xian-Yan, PANG Ming-Li, ZHAO Qiang, REN Yi-Ran, HAO Yu-Jin, YOU Chun-Xiang

State Key Laboratory of Crop Biology，National Research Center for Apple Engineering and Technology，College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China

Online:2015-07-25 Published:2015-07-25

Abstract

Abstract: Laccases are glycoproteins with polyphenol oxidase acitivity depending on copper. They ubiquitously exist in plants，fungi，insects，bacteria and other organisms，playing crucial roles in lignin synthesis，ion absorption and stress responses. It has been documented that several LAC genes were regulated by microRNAs at post–transcriptional level. In this study，homology BLAST was conducted in GenBank for LAC ESTs. Resultly，15 tomato ESTs highly similar with LAC genes were obtained，and spliced into a LAC fragment named as LeLACmiR397 containing a recognition site of miR397. In addition，a Cu-oxidase domain was predicted to be in the deduced LeLACmiR397 protein. To check the temporal and spatial expression of LeLACmiR397，semi-quantitative RT-PCR was performed. It was found that LeLACmiR397 specifically expressed in roots，flowers，ripe fruit and callus，but not in leaves. Then，its full length cDNA was cloned with RT-PCR and 5′- and 3′-RACEs，and registered as EU503151 in GenBank，corresponding to Solyc07g049460.2.1 in tomato genome. The data showed that LeLACmiR397 expression was down-regulated in the transgenic tomato overexpressing miR397a，and the contents of PPO，POD and SOD were reduced too. When being treated with Syringic acid and Sinapic acid，transgenic tomatoseedlings overexpressing LeLACmiR397 had longer root system and showed stronger resistance than WT. These results suggested that LeLACmiR397 was related to resistance to Syringic acid and Sinapic acid in tomato.

Key words: tomato, laccase, LAC, cloning, LeLACmiR397gene, resistance

CLC Number:

S 641.2

ZHAO Xian-Yan, PANG Ming-Li, ZHAO Qiang, REN Yi-Ran, HAO Yu-Jin, YOU Chun-Xiang. Cloning and Expression Analysis of Tomato LeLACmiR397 Gene[J]. ACTA HORTICULTURAE SINICA, 2015, 42(7): 1285-1298.

[1]	WANG Xiqing, JIA Yunhe, YAN Wen, FU Yongkai, YOU Haibo, LI Dongyan, and ZHAO Jingchao. A New Watermelon Cultivar‘Longsheng Jiali’with High Resistance to Fusarium Wilt [J]. Acta Horticulturae Sinica, 2023, 50(2): 455-456.
[2]	SHI Hongli, LI La, GUO Cuimei, YU Tingting, JIAN Wei, YANG Xingyong. Isolation，Identification and Analysis of Biocontrol Ability of Biocontrol Strain TL1 Against Tomato Botrytis cinerea [J]. Acta Horticulturae Sinica, 2023, 50(1): 79-90.
[3]	HU Jingyu, QUE Kaijuan, MIAO Tianli, WU Shaozheng, WANG Tiantian, ZHANG Lei, DONG Xian, JI Pengzhang, DONG Jiahong. Identification of Tomato Spotted Wilt Orthotospovirus Infecting Iris tectorum [J]. Acta Horticulturae Sinica, 2023, 50(1): 170-176.
[4]	LI Zhiliang, LI Zhenxing, LI Tao, SUN Baojuan, LI Ying, XU Xiaowan, WANG Hengming, HENG Zhou, GONG Chao. A New Eggplant Cultivar‘Xinfeng 3’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 227-228.
[5]	YANG Xingwang, WANG Haibo, WANG Yingying, WANG Xiaolong, WANG Zhiqiang, LIU Peipei, LIU Wanchun, and WANG Xiaodi. A New Mid-ripening and Cold Resistant Peach Cultivar‘Zhongnong Ganshuang’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 15-16.
[6]	YANG Xingwang, WANG Haibo, WANG Yingying, ZHANG Yican, WANG Baoliang, Liu Peipei, SHI Xiangbin, LIU Wanchun, and WANG Xiaodi. A New Mid-ripening Cold Resistant Peach Cultivar‘Zhongnong Baigan’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 17-18.
[7]	YANG Xingwang, LIU Fengzhi, WANG Haibo, WANG Yingying, WANG Zhiqiang, SHI Xiangbin, JI Xiaohao, LIU Wanchun, and WANG Xiaodi. A New Mid-ripening Cold Resistant Peach Cultivar‘Zhongnong Hanshuimi’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 19-20.
[8]	YANG Xingwang, LIU Fengzhi, WANG Haibo, WANG Yingying, ZHANG Yican, LI Peng, WANG Xiaolong, LIU Wanchun, and WANG Xiaodi. A New Late-ripening Cold Resistant Peach Cultivar‘Zhongnong Qiuxiang’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 21-22.
[9]	QI Yongjie, GAO Zhenghui, MA Na, WANG Qingming, KE Fanjun, CHEN Qian, and XU Yiliu, . A New Yellow Flesh and Resistant to Canker Disease Kiwifruit Cultivar ‘Wannong Jinguo’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 49-50.
[10]	YU Yangjun, WANG Weihong, SU Tongbing, ZHANG Fenglan, ZHANG Deshuang, ZHAO Xiuyun, YU Shuancang, LI Peirong, XIN Xiaoyun, and WANG Jiao. A New Chinese Cabbage Cultivar‘Jingchun CR3’with Clubroot Resistance and Bolting Tolerance [J]. Acta Horticulturae Sinica, 2022, 49(S2): 87-88.
[11]	WANG Lili, WANG Xin, WU Haidong, WEN Qiang, and Yang Xiaofei. A New Chinese Cabbage Cultivar‘Liaobai 28’with Resistance to Clubroot Disease [J]. Acta Horticulturae Sinica, 2022, 49(S2): 89-90.
[12]	LIU Zhiyuan, XU Zhaosheng, ZHANG Helong, and QIAN Wei. A New Oversummer Spinach Cultivar‘Shubo 16’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 95-96.
[13]	HUANG Shuping, TAN Jie, Chen Xia, ZHANG Hongyuan, LI Ye, WANG Benqi, CHEN Hao, WU Xuexia, and ZHANG Min, . A New Eggplant Cultivar‘Eqie 6’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 101-102.
[14]	ZHENG Jirong, WANG Tonglin, and HU Songshen. A New Tomato Cultivar‘Hangza 603’with High Quality [J]. Acta Horticulturae Sinica, 2022, 49(S2): 103-104.
[15]	ZHENG Jirong and WANG Tonglin. A New Tomato Cultivar‘Hangza 601’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 105-106.

Cloning and Expression Analysis of Tomato LeLACmiR397 Gene

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments