Abstract: Rubus is a large and taxonomically complex group that displays agamospermy，polyploidy and frequent hybridization. The phylogeny of the genus has long been controversial. It is also difficult to identify and classify the wild excellent Rubus germplasms. The chloroplast DNA fragments ndhF of 53 Rubus species（variety）were analyzed for phylogenetic relationships among the genus by the Maximum parsimony（MP）and Bayesian inference（BI）analysis. Rosa xanthina and Fragaria vesca were used as outgroups，which were related to Rubus and belonged to Rosoideae subfamily. The results revealed that the ndhF among the genus was relatively conserved and 1182 bp in length. There were 88（7.44%）variable sites and 47（3.97%）parsimony information sites. The sequence divergence in section Idaeobatus was faster than that in sections Malachobatus and Dalibardastrum. Multiple DNA regions evolved rapidly should be used to resolve phylogenetic relationships within sect. Malachobatus. The phylogenetic tree indicated that those species from sections Malachobatus and Dalibardastrum formed a well-supported clade，whilethe species of sect. Idaeobatus were divided into four clades and polyphyletic. Based on the data，it was inferred that evolution in Rubus proceeded from compound leaf to simple leaf，and sect. Idaeobatus were the primitive group. These results supported the evolution view of Lu. It is helpful to identify and classify the wild excellent Rubus germplasms，as well as accumulate molecular data for the phylogeny of Rubus.

Key words: Rubus, sect. Idaeobatus, sect. Malachobatus, ndhF, phylogenetic utility