园艺学报 ›› 2016, Vol. 43 ›› Issue (7): 1305-1314.doi: 10.16420/j.issn.0513-353x.2016-0159

• 蔬菜 • 上一篇    下一篇


赵丽玲,钟 静,尹跃艳,丁 铭*,张仲凯*   

  1. (云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,昆明 650223)
  • 出版日期:2016-07-25 发布日期:2016-07-25

Recombination and Mixed Infection of Two Distinct Begomoviruses in Tomato

ZHAO Li-ling,ZHONG Jing,YIN Yue-yan,DING Ming*,and ZHANG Zhong-kai*   

  1. (Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Key Laboratory of Agricultural Biotechnology of Yunnan Province,Kunming 650223,China)
  • Online:2016-07-25 Published:2016-07-25



关键词: 菜豆金色花叶病毒属病毒, 番茄曲叶病, beta卫星, 复合侵染, 重组


Tomato leaf curl disease induced by begomoviruses pose a serious constraint to tomato production worldwide. Mixed infections in natural condition may cause recombination or pseudorecombination events and generated new virus strains,species or diseased complex that either intensify or ameliorate the severity of disease. In this study,two begomovirus isolates Y3080-32 and Y3080-40,and two betasatellite isolates Y3080-1 and Y3080-2,were obtained from a diseased tomato plant in Honghe,Yunnan Province,China,which shows leaf yellowing and stunting symptoms. Sequence comparison shows that Y3080-32 is highly related to Malvastrum yellow vein Honghe virus(MaYVHoV)that was reported previously to infect only Malvastrum coromandelianum,while Y3080-40 belongs to Pepper leaf curl Yunnan virus(PepLCYnV). Recombination analysis reveals that the isolate Y3080-32 is  a recombinant begomovirus derived from MaYVHoV and PepLCYnV(Y3080-40). In addition,this study shows that the betasatellite isolates of Y3080-1 and Y3080-2 are isolates of Malvastrum yellow vein betasatellite(MaYVB). This report is a documentation of co-infection of MaYVHoV/MaYVB disease complex and PepLCYnV in tomato,and indicates mixed begomovirus infections provide opportunities for recombination and pseudorecombination events to occur.

Key words: begomoviruses, tomato leaf curl disease, betasatellite, mixed infection, recombination