园艺学报 ›› 2013, Vol. 40 ›› Issue (6): 1110-.

• 观赏植物 • 上一篇    下一篇


刘 芳1,2,王家艳1,王晓丽3,周蕴薇1,*   

  1. (1东北林业大学园林学院,哈尔滨 150040;2黑龙江八一农垦大学农学院,黑龙江大庆 163319;3呼伦贝尔市蚕业科学研究所,内蒙古呼伦贝尔 162650)
  • 收稿日期:2012-12-04 修回日期:2013-05-06 出版日期:2013-06-25 发布日期:2013-06-25

The Apical Bud Cell Ultra-structure Changes of Lilium pumilum Bulbs During Breaking Dormancy Under Refrigerated Condition

LIU Fang1,2,WANG Jia-yan1,WANG Xiao-li3,and ZHOU Yun-wei1,*   

  1. (1College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China;2College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;3The Hulunbuir Sericultural Science Research Institute,Hulunbuir,Inner Mongolia 162650,China)
  • Received:2012-12-04 Revised:2013-05-06 Online:2013-06-25 Published:2013-06-25

摘要: 研究了细叶百合鳞茎低温处理打破休眠过程中的形态变化及茎尖细胞超微结构的变化。结果表明,低温处理0 ~ 12 d,鳞茎物质和能量代谢较弱,鳞茎不能萌发;12 ~ 48 d,鳞茎萌发率较低,线粒体及高尔基体分泌囊泡增多,细胞通过质膜内吞及胞间连丝进行物质交流;48 ~ 72 d,核仁松散,内质网有序排列,在液泡膜与质膜边缘形成内质网桥,内质网桥一直延伸到核膜方向,胞内及胞间联系已经建立;出芽时,内质网桥延伸到胞间连丝,细胞器内部结构及数量增加,休眠完全打破。

关键词: 百合, 鳞茎, 冷藏, 茎尖细胞, 超微结构

Abstract: The bulb apical bud cell ultra-structure changes of Lilium pumilum bulbs during breaking dormancy under refrigerated condition were studied. Results showed that the material and energy metabolism of bulbs were weak between 0 to 12 days and bulbs couldn’t sprout;The germination rate of bulbs was lower and mitochondria and golgi vesicles increased,cells exchanged materials through the plasma membrane endocytosis and plasmodesmata between 12 to 48 days. Nucleoli were incompact and endoplasmic reticulums scattered orderly between 48 to 72 days,and endoplasm bridges formed at the edges of vacuole membrane and plasma membrane,with some of them extending to the nuclear envelope. Intracellular and intercellular connection had been established in this period. Endoplasm bridges and the plasmodesmata connected in budding period,and the internal structures and quantity of organelles increased obviously,dormancy is completely broken in this period.

Key words: lily;bulb, cold storage, apical bud cell, ultrastructure