园艺学报 ›› 2013, Vol. 40 ›› Issue (3): 571-578.

• 研究报告 • 上一篇    下一篇

南方红豆杉SSR 分布特征分析及分子标记的开发


  1. (1 浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安 311300;2 宁波城市职业技术学院,浙江奉化
  • 出版日期:2013-03-25 发布日期:2013-03-25

SSR Distribution Characteristic Analysis and Molecular Marker Development
in Taxus wallichiana var. mairei

 YI  Guan-Mei, LI  Jian-Hui, WANG  Dong-Mei, TONG  Zai-Kang, LU  Yong-Quan   

  1. (1The Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang Agricultural and Forestry
    University,Lin’an,Zhejiang 311300,China;2Ningbo City College of Vocational Technology,Fenghua,Zhejiang 315502,
  • Online:2013-03-25 Published:2013-03-25

摘要: 利用基因组勘测序列(GSS 序列)探讨开发南方红豆杉(Taxus wallichiana var. mairei)SSR
标记的可行性。从1 923 条GSS 序列中搜寻到184 个SSR 位点。其中二核苷酸、三核苷酸和四核苷酸重
复所占比例分别为88.6%、10.3%和1.1%。不同核苷酸在重复序列中的频率分析表明,a 和t 所出现的频
率远远高于c 和g 的频率。对所获得的184 个SSR 位点设计引物,开发出90 个候选SSR 引物。在所获
得的获选标记中选取20 对引物在南方红豆杉中进行试验检验,结果19 对(95%)SSR 引物能够获得清晰
稳定的主带,表明GSS 序列可以高效地用于开发基因组SSR 标记,这些标记将为南方红豆杉的相关研究

关键词: 南方红豆杉, GSS 序列, SSR 标记, 频率

Abstract: Abstract:In order to provide tool of biology research and genetic improvement for Taxus wallichiana
var. mairei,SSR molecular markers were developed with GSS sequence in this study. In total 184 SSR loci
were find from 1 923 GSS sequences. Among those loci,the proportion of two nucleotide repeats,three
nucleotide repeats and four nucleotide repeats are 88.6%,10.3%,1.1%,respectively. The frequency of a
and t are higher than that of c and g in the nucleotide repeats in the T. wallichiana var. mairei genome.
From those 184 SSR loci,90 SSR candidate primers were exploited. In order to verify the validity of
candidate primers,20 primers were selected for further test. PCR result showed that 19 can gain clear
products. Those results showed that GSS data could be employed to exploit SSR marker. Those markers
can be well used in T. wallichiana var. mairei research.

Key words: Taxus wallichiana var. mairei, GSS sequence, SSR, frequency