园艺学报 ›› 2013, Vol. 40 ›› Issue (3): 515-522.

• 观赏植物 • 上一篇    下一篇

牡丹柱枝孢叶斑病(Cylindrocladium canadense)对叶片光合系统功能的影响

 杨德翠, 刘 超, 盖树鹏, 郑国生, 郭平毅   

  1. (1 山西农业大学农学院,山西太谷 030801;2 青岛农业大学生命科学学院,山东省高校植物生物技术重点实验室,
    山东青岛 266109)
  • 出版日期:2013-03-25 发布日期:2013-03-25

Effect of Infection by Cylindrocladium canadense on Behaviors of
Photosystems in Tree Peony Leaves

 YANG  De-Cui, LIU   Chao, GAI  Shu-Peng, ZHENG  Guo-Sheng, GUO  Ping-Yi   

  1. (1Agronomy College,Shanxi Agricultural University,Taigu,Shanxi 030801,China;2College of Life Sciences,Qingdao
    Agricultural University,Key Lab of Plant Biotechnology in Universities of Shangdong Province,Qingdao,Shandong
  • Online:2013-03-25 Published:2013-03-25

摘要: 以牡丹(Paeonia suffruticosa)‘藏枝红’为试验材料,通过测定叶绿素荧光快速诱导动力学
曲线、820 nm 光吸收曲线以及气孔交换参数和H2O2 含量,研究了柱枝孢叶斑病(Cylindrocladium
速率(Pn)、气孔导度和叶绿素含量均下降,而细胞间隙CO2 浓度升高,表明Pn 的降低是受非气孔因素的
影响。Cylindrocladium canadense 的侵染,使H2O2 含量显著升高,快速叶绿素荧光诱导动力学曲线形状
发生明显改变,F v/F m 和PIABS 均显著下降。PSⅡ供体侧、反应中心和受体侧活性均受到抑制,但PSⅡ受
Cylindrocladium canadense 的侵染,使H2O2  积累增加,对PSⅠ和PSⅡ的功能造成伤害。PSⅠ活性的下降
阻碍了PSⅡ向PSⅠ的电子传递,过剩激发能增加,导致ROS 增加,抑制D1 蛋白的合成,加剧了对PSⅡ

关键词: 牡丹, 柱枝孢叶斑病, 光系统Ⅰ, 光系统Ⅱ, 活性氧

Abstract: In the present study,Effect of infection by Cylindrocladium canadense on behaviors of
photosystemⅠ(PSⅠ)and photosystemⅡ(PSⅡ)in tree peony(Paeonia suffruticosa‘Zangzhihong’)
leaves was estimated by simultaneously measuring their Chlorophyll fluorescence transient,light
absorbance at 820 nm,gas exchange and hydrogen peroxide(H2O2)content. The data showed that the net
photosynthetic rate(P n),stomatal conductance and chlorophyll content were significantly decreased
compared with that of the control after infection by C. canadense,whereas intercellular CO2 concentration
was significantly increased. These results indicated that the decreased photosynthesis in infected leaves
was resulted from non-stomatal factor. Furthermore,significantly enhanced H2O2 content,reduced Fv/Fm
and PIABS,as well as the changed chlorophyll fluorescence transient was also observed in leaves of tree
peony infected by C. canadense. The donor sides(Oxygen evolution complex),reaction centers and
acceptor sides of PSⅡ were significantly inhibited by C. canadense infection. But the acceptor sides were
more suffered than the donor sides. At the same time,the activity of PSⅠ was also significantly reduced.
Taken together,the data presented here indicated that C. canadense infection resulted in the accumulation
of H2O2,which in turn damaged the functions of PSⅠand PSⅡ. The decline of PSⅠ activity inhibited
transportation of electrons from PSⅡ to PSⅠ,which then led to the accumulation of excessive excitation
energy and reactive oxygen species(ROS). The increased ROS Inhibited synthesis of protein D1,
accelerating damage to PSⅡ. It was the main reason for inhibition of photosystems in tree peony leaves
after infected by C. canadense.

Key words: tree peony, Cylindrocladium canadense, PSⅠ, PSⅡ, reactive oxygen species