关键词: 平榛, ChDHN, qRT-PCR, 原核表达

Abstract: A cDNA encoding the dehydrin-like gene homologue was isolated from hazelnut（Corylus heterophylla Fisch.）by RACE-PCR and designated ChDHN（GenBank accession No. HM228389）. Sequence analysis showed that cDNA of ChDHN was 639 bp long and contained a single open reading frame. The predicted ChDHN protein has 167 amino acids with an estimated molecular mass of 18.03 kD and an isoelectric point of 7.28，qRT-PCR analysis showed that the expression of ChDHN was induced by low temperature and peaked at 24 h after exposed to low temperatures of 4 ℃. The transcripts of ChDHN appeared in many hazelnut tissues including male inflorescence，bark，flower bud and seeds，but mostly accumulated in seeds. the prokaryotic expression plasmid of pET-32a(+)-DHN was sequenced，digested by restricted endonuclease enzyme of SacⅠand EcoRⅠ simultaneously，meanwhile induce it to be expressed in E. coli BL21 by IPTG，SDS-PAGE analysis and Western blot results showed that the recombinant plasmid pET-32a(+)-His-DHN could be expressed a 4 kD larger molecule mass than predicted molecule mass of fusion protein.

Key words: hazelnut, ChDHN, qRT-PCR, prokaryotic expression