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园艺学报 ›› 2003, Vol. 30 ›› Issue (02): 204-206.

• 研究报告 • 上一篇    下一篇

刺梨及部分近缘种形态学性状和RAPD标记分析

文晓鹏1,2;庞晓明1; 邓秀新1*
  

  • 收稿日期:2002-04-24 修回日期:2002-07-08 出版日期:2003-04-25 发布日期:2003-04-25

A Comparative Study of RAPD and Morphological Approaches to CharacterizeRelationships of Rosaroxburghii Tratt.and Its Relatives

Wen Xiaopeng1,2; PangXlin1; Deng Xiuxin1*
  

  1. (1 National Key Laboratory of Crop Genetic Improvement, Hangzhou , 2Agriculture University, Wuhan 430070, China;2 Agirculture College,Guizhou University, Guiyang 550025,China)
  • Received:2002-04-24 Revised:2002-07-08 Online:2003-04-25 Published:2003-04-25

摘要: 通过36个形态学性状和29个引物RAPD扩增获得的分子标记,探索刺梨及部分近缘种的亲缘
关系。结果表明,普通刺梨所有基因型之间的亲缘关系较近,而与其余种类相对较远;无籽刺梨与贵州缫丝花的遗传距离较近,形态学和RAPD聚类分析都显示很近的亲缘关系;尽管两类遗传距离的排序和聚类分析有一定差异,但Mantel相关性检测表现出显著相关性(r=0.6122,P<0.01)。还就无籽刺梨的可能来源进行了讨论。

关键词: 刺梨, 近缘种, 形态学, RAPD, Mantel检测

Abstract: This sixty morphological traits and RAPD markers amplified with 29 arbitrary primer used to discriminate the accessition, and to evaluate the relatedness of Rosa roxburghii Tmtt.and a sample set of its relatives.Compared with the relatives,considerable close relationships were found among the genotypes of R.burghii Tmtt.Furthermore,a hish relatedness was also obtained between R.S.Shi and D. Shi and R. kweichowensis Yu et Ku.A Mantel correspondence test showed a significant correlation (r=0.6122,P<0.01)between morphological traits and markers,despite certain discontance existing in the two approaches. In addition,the origination of R.sters S.D. was discussed in the paper.

Key words: Rosa roxburghii Tratt., Relatives, Morphology, RAPD, Mantel correspondence test

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