一氧化氮和过氧化氢对地被菊扦插生根的影响

园艺学报 ›› 2009, Vol. 36 ›› Issue (11): 1643-1650.

一氧化氮和过氧化氢对地被菊扦插生根的影响

廖伟彪^1,2;张美玲^3,4*;吴永华⁵;肖洪浪²

(¹甘肃农业大学农学院, 甘肃省作物遗传改良与种质创新重点实验室, 兰州730070; ²中国科学院寒区旱区环境与工程研究所, 兰州730000; ³甘肃农业大学草业学院, 兰州730000; ⁴甘肃农业大学理学院, 兰州730000; ⁵兰州市园林科学研究所, 兰州730070)

收稿日期:2009-04-30 修回日期:2009-09-17 出版日期:2009-11-25 发布日期:2009-11-25
通讯作者: 张美玲

Effects of Nitric Oxide and Hydrogen Peroxide on Rooting of Ground-coverChrysanthemum Cuttings

LIAO Wei-biao^1,2;ZHANGMei-ling^3,4*;WU Yong-hua⁵;XIAO Hong-lang²

(¹ College of Agronomy, Gansu Agricultural University, Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Lanzhou 730070, China; ²Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730070, China; ³College of Prataculture, Gansu Agricultural University, Lanzhou 730070, China; ⁴College of Science, Gansu Agricultural University, Lanzhou 730070, China; ⁵ Institute of Garden Research of Lanzhou, Lanzhou 730070, China)

Received:2009-04-30 Revised:2009-09-17 Online:2009-11-25 Published:2009-11-25
Contact: ZHANGMei-ling

摘要/Abstract

摘要： 以地被菊(Dendranthema morifolium ) 品种‘北国之春’为试验材料, 研究了一氧化氮(NO)和过氧化氢(H₂O₂ ) 对其插穗生根的影响以及生根过程中插穗叶片超氧化物岐化酶(SOD) 、过氧化物酶( POD) 、多酚氧化酶( PPO) 活性以及酚类物质含量的变化。结果表明, 外源NO和H₂O₂可促进地被菊插穗生根以及根的生长, 且表现出明显的浓度效应。促进生根最适硝普纳( SNP, NO供体) 和H₂O₂的浓度分别为50和200μmol·L^-1。同时, SNP和H₂O₂共同处理的地被菊插穗生根率显著高于SNP或H₂O₂单独处理, 说明NO和H₂O₂在地被菊插穗生根过程中具有协同诱导效应。另外, 相比对照, SNP、H2O2和两者共同处理插穗叶片的SOD和PPO活性显著提高。而SNP、H₂O₂和两者共同处理比对照分别具有更低的POD活性和酚类物质含量, 且SNP +H₂O₂处理的POD活性和酚类物质含量低于SNP或H₂O₂单独处理。综合以上结果可知, 50μmol·L^-1 SNP和200μmol·L ^-1H₂O₂处理可以提高插穗叶片SOD和PPO活性, 降低POD的活性和酚类物质的含量, 从而促进插穗生根和根的生长。

关键词: 地被菊, 一氧化氮(NO), 过氧化氢(H₂O₂), 插穗, 生根

Abstract: Ground2cover chrysanthemum (Dend ranthem a m orifolium‘Beiguozhichun’) was used to understand the effects of nitric oxide (NO) and hydrogen peroxide (H₂O₂ ) on rooting of p lant cuttings and the changes of activities of superoxide dismutase ( SOD) , peroxidase (POD) and polyphenol oxidase ( PPO) and the total polyphenol content of cutting leaves. The results showed that the effects of exogenous H₂O₂ or NO on rooting of ground-cover chrysanthemum cuttings was dose dependent, with a maximal biological response at 200μmol·L ^-1H₂O₂ or 50μmol·L ^-1 NO donors SNP. At the same time, the rooting percentage of cuttings treated with both SNP and H₂O₂ was significantly higher than that of cuttings treated with SNP or H₂O₂ alone,
which suggested that there might exist synergistic action between H₂O₂ and NO on mediating rooting. Additionally, compared with the control, SNP, H₂O₂ and SNP +H₂O₂ treatmentsmight significantly increase the activities of SOD and PPO of ground-cover chrysanthemum cuttings leaves. However, the activities of PODand the total polyphenol content were significantly higher in control than in other treatments during rooting. Moreover, lower POD activities and polyphenol contentwere attained in SNP +H₂O₂ treatment than in SNP or
H₂O₂ treatment alone. Together, these results indicated that 50μmol·L ^-1 SNP and 200μmol·L - 1 H₂O₂treatments enhanced rooting and root growth synergistically and independently by stimulating the activities of SOD and PPO enzymes, and simultaneously by rep ressing POD activities and the p roduction of polyphenol.

Key words: ground-cover chrysanthemum, nitric oxide (NO), hydrogen peroxide (H₂O₂ ), cuttings, rooting

中图分类号:

S 682.1⁺1

廖伟彪;张美玲;吴永华;肖洪浪. 一氧化氮和过氧化氢对地被菊扦插生根的影响[J]. 园艺学报, 2009, 36(11): 1643-1650.

LIAO Wei-biao;ZHANGMei-ling;WU Yong-hua;XIAO Hong-lang. Effects of Nitric Oxide and Hydrogen Peroxide on Rooting of Ground-coverChrysanthemum Cuttings[J]. ACTA HORTICULTURAE SINICA, 2009, 36(11): 1643-1650.

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